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技術(shù)文章您的位置:網(wǎng)站首頁(yè) >技術(shù)文章 > 清華大學(xué)免疫學(xué)醫(yī)學(xué)院轉(zhuǎn)染BMDMs細(xì)胞發(fā)表文章到JEM已見(jiàn)刊
清華大學(xué)免疫學(xué)醫(yī)學(xué)院轉(zhuǎn)染BMDMs細(xì)胞發(fā)表文章到JEM已見(jiàn)刊
更新時(shí)間:2020-06-15   點(diǎn)擊次數(shù):1189次

一、2020一季度,清華大學(xué)免疫學(xué)與醫(yī)學(xué)院;清華大學(xué)生命科學(xué)學(xué)院聯(lián)合發(fā)表標(biāo)題為:The colonic macrophage transcription factor RBP-J orchestrates intestinal immunity against bacterial pathogens的文章到JEM;

二、用invigentech(英克)INVI DNA RNA Transfection Reagent轉(zhuǎn)染試劑;

三、成功轉(zhuǎn)染BMDMs細(xì)胞(小鼠骨髓來(lái)源的巨噬細(xì)胞)發(fā)表文章見(jiàn)刊;

四、文章簡(jiǎn)介:結(jié)腸巨噬細(xì)胞轉(zhuǎn)錄因子RBP-J協(xié)調(diào)腸道對(duì)細(xì)菌的免疫病原體研究.

五、文章部分內(nèi)容見(jiàn)下:

RNA-mediated interference

siRNA specifically targeting mouse Cebpb and nontargeting control siRNA were purchased from GenePharma Company. siRNA was transfected into BMDMs using INVI DNA RNA Transfection Reagent according to the manufacturer’s in structions (Invigentech). Cells were used 48 h after transfection. 

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